Laboratory

Thermo Electron DECA-LCQ
LDP00200
Electrospray, nanospray, and APCI ionization – quadrupole ion trap.
~1000 mass resolution

The DECA-LCQ XP’s ionization techniques are soft, and it has a mass range of 15-2000 m/z. It has capabilities of MSn (n=2-10).
Atmospheric Pressure Chemical Ionization (APCI)- gas phase ionization technique, used to analyze small molecules that have some volatility and medium polarity.
Electrospray Ionization Mode(ESI)- used to analyze any polar compound that can ionize in solution. It is used to look at biological polymers, industrial polymers and pharmaceuticals. 1uL/min – 1000 uL/min flow rates.
Nano-Electrospray- low flow rates 10-40 nL/min, low sample volumes down to 300nL, less contamination to the instrument and less sample cleanup is needed.

 

 
Jobin Yvon Horiba Triple Grating Raman with Olympus BX40 Optical Microscope
N001185
T 64000 Triple additive Raman Spectrometer
~0.1 wavenumber resolution

The second laboratory consists of a triple grating Raman scattering spectrometer. This instrument is fitted with 4 laser sources (488, 514, 532, and 631nm) and a mercury lamp. We will begin using this equipment to look at protein binding studies, and purity of sample.
PerSeptive Biosystems Voyager-DE Biospectrometry™ Workstation
Model 5-2386-00
Matrix-assisted laser-desorption ionization – time-of-flight mass spectrometer
~5000 mass resolution

This instrument is capable of high sensitivity allowing for small sample sizes of 5 femtomoles using the dried droplet application technique. It can analyze mixtures of proteins, synthetic polymers, oligonucleotides and other biopolymesrs of interest. It is capable of obtaining a complete mass spectrum from a single ionization event known as multiplexing. It has a mass range in excess of 300 KDa m/z and it uses a nitrogen laser at 337nm.
Zyvex – 4 positioner nanomanipulator with Nikon TE2000U inverted microscope
L200
Nanomanipulator with 2 probes and 2 capillary for nanoscale analysis
~500nm resolution

The nanomanipulator has two modes in the X, Y, and Z axis a fine mode (100 microns of travel in the X and Z axis and 10 microns of travel in the Y axis with 5nm resolution) and the coarse mode (12 mm of travel in the X and X axis and 28mm of travel in the Y axis with100nm resolution). It is controlled by a joystick for easy handling. It is mounted on the inverted microscopes stage. Its applications are direct sampling of biological and forensic samples, electrophysiology of cells, biomaterial characterization, and electrical characterization of micro- and nanoscale biomaterials
The Nikon microscope had 4 objectives a 10X, two 40X, and 100X capable of bright field, phase contrast, DIC, and fluorescence. It has cameras that are capable of taking still images and video recordings.